The 6X His-tag (C-terminal) antibody is a non-conjugated, mouse-originated IgG2a monoclonal antibody designed for research use. It targets the 6X His-tag, a common affinity tag of six histidine residues that binds to affinity chromatography media like nickel or cobalt, making it useful for protein purification.
- Host: mouse
- Clonality: monoclonal
- Molecular weight: predicted 30 kDa; observed 32 kDa
- Species reactivity: species independent
- Immunogen: synthetic peptide corresponding to His-tag
- Conjugation: non-conjugated
- Modification: unmodified
- Isotype: IgG2a
- Appearance: liquid
- Formulation: supplied in PBS (pH 7.4), 0.1% BSA, 40% Glycerol with 0.05% Sodium Azide as preservative
- Storage & stability: store at -20°C for 1 year; avoid repeated freeze/thaw cycles
- Shipping: shipped with blue ice
- Applications: WB (1:5000-1:20000), IP (assay dependent concentration)